Invitro analysis of seed germination parameter in blackgram(Vigna mungo L. Hepper ) by using Polyethylene glycol (PEG)

Abstract

The present invitro study carried out with eleven blackgram genotypes including T-9 used as check were collected from NBPGR regional centre Hyderabd and ICAR-CRIDA Hyderabad respectively. Seeds that were healthy and uniformly sized were surface sterilized for two to three minutes using 0.1% mercuric chloride (HgCl2) before being thoroughly washed with distilled water. The sterilized (10) seeds were sown in petri plates with moistened germination paper and various water potentials, namely, 0.0 (control), 5%, 10%, 15%, 20%, 25% and 30% of PEG 6000 in 100 ml of distilled water. The ANOVA results revealed sgnificant variability due to treatment, genotype and treatment verses genotype for the trait seed grmination at all the percentage of PEG 6000 studied. The results showed that among eleven genotypes IC426766 and IC382811 recorded higher germination percentage at higher PEG concentration and upto 20% PEG very lower percentage of germination was observed. It indicates that the variety IC426766 with hold the water even at higher concentration and showing water stress condition.